Dr. Nguyen Thi Mai Phuong

PERSONAL INFORMATION

• Name: Nguyen Thi Mai Phuong

• Academic title: PhD, Senior researcher

• Position: Vice-Head of Department

• Telephone: 04-38360853

• Email: phuong_nguyen_99@yahoo.com

I. EDUCATION AND TRAINING

1988: BSc. in Biological Sciences (Biochemistry); Hanoi University of Science

1997: Master of Science in Biotechnology (Plant Molecular Biology); University of Wollongong, Australia.

1998: Honours Master of Science in Biotechnology (Plant Molecular Biology); University of Wollongong, Australia.

2005: Ph.D in Biological Sciences (Biochemistry) Institute of Biotechnology, Vietnamese Academy of Sciences and Technology, Caugiay-Hanoi, Vietnam.

Other qualification/Work experience

• 1999 - 2001: Visiting fellow at of Microbiology and Immunology, University of Rochester Medical Center, USA.

• 2003-2004: Visiting scientist at Department of Microbiology and Immunology, University of Rochester Medical Center, USA.

• 5/ 2006 - 7/2006: Participant of "Bioindustry " training course organized by Japanese International Cooperation Agency (JICA), Japan.

• 2008 - 2009: Postdoctoral Research Associate at Department of Microbiology and Immunology, University of Rochester, New York, USA.

• 2013 - 2014: Postdoctoral Research Associate at Center for Oral Biology, University of Rochester, New York, USA (Vietnam Education Foundation grant).

• 9/2014 12/2014: Postdoctoral Research Associate at Department of Chemistry, University of Mahidol, Thailand (NSRC-IFS Fellowship)

• 7/2015 – 8/2015: Postdoctoral Research Associate at Institute of Pharmacy, University of Greisfwald, Germany ( DAAD Fellowship)

• 10/2015 - 11/2015: Postdoctoral Research Associate at Department of Pharmacy and Pharmacology, University of Bath, UK (British Council Newton's grant)

II. CURRENT POSITION

1989 - present time (2015): Senior researcher at the Department of Plant Biochemistry, Institute of Biotechnology, Vietnamese Academy of Sciences and Technology, Caugiay, Hanoi, Vietnam.

III. FIELDS OF CURRENT RESEARCH/INTEREST

• Biochemistry of proteins/enzymes, bio-active compounds and application.

• Assembly principles and the virulence determinants of biofilms, as well as developing novel therapeutic approaches to prevent biofilm-dependent infectious diseases.

RESEARCH FUNDINGS

2000 – 2005: "Study and application of bioactive preparations from naturally-occurring plants with medicinal potentially use for treatment of common diseases in Vietnam"; Ministry of Science and Technology; Grant Investigator.

2005-2011: "Oxidative damage in oral streptococci"; National Institute for Dental and Craniofacial Research, USA; Grant Investigator.

2006-2008: "New antimicrobial agents from the plants of Vietnam: improved protection against dental caries" - (F4087/1); Internatonal Foundation for Science (IFS), Sweden; Principle investigator.

2006-2008: "Screening for new antimicrobial peptides from Vietnamse bioresources"; Institute of Biotechnology, Vietnamese Academy of Science and Technology; Principle investigator.

2006 – 2009: "Screening and mode of actions of agents against dental caries"; Ministry of Science and Technology, Vietnam; Principle investigator.

2010 – 2011: "Production of mouthwash containing alpha-mangostin isolated from the peel of mangosteen (Garciana mangostana L.) grown in Vietnam"; Institute of Biotechnology, Vietnamese Academy of Science and Technology; Principle investigator.

2012 – 2014: "New antimicrobial agents from the plants of Vietnam: improved protection against dental caries" - (F4087/2); Internatonal Foundation for Science (IFS), Sweden; Principle investigator.

2012: "Production of synbiotics from bacterial spores and rice bran"; Ministry of Science and Technology, Vietnam KC04.TN01/11-15; Principle investigator.

2012-2013: "Establishment of screening systems for novel anti-inflammatory agents using macrophage membrane bound glucocorticoid and toll-like family receptor"; Vietnam Academy of Science and Technology, Vietnam - VAST03; Principle investigator.

2011-2015: "Mechanisms of actions of phytochemicals against Streptococus mutans in biofilms"; National Foundation for Sciences and Technology (NAFOSTED); Principle investigator.

2014-2016: "Development of polymeric nanoparticle encapsulated mangostin (nanomangostin): a new approach to treating biolilm diseases"; Third World Academy of Science (TWAS); Italy; Principle investigator.

IV. PUBLICATIONS

1. *Phuong Thi Mai Nguyen, Bac Hoai Vo, Nhung Thi Tran, Quyen Dong Van (2015) Anti-biofilm activity of α-mangostin isolated from Garcinia mangostana L. Z Naturforsch. 70(11-12)c:313–318 doi: 10.1515/znc-2015-0187.

2. Nguyen Thi Mai Phuong, Vo Hoai Bac, Tran Thi Nhung, Do Hoang Hiep (2015) Preparation of protein isolates from rice bran. J Biol (In press).

3. Ngo Van Quang, Nguyen Xuan Nhiem, Dan Thi Thuy Hang, Hoang Le Tuan Anh, Pham Hai Yen, Duong Thi Dung, Nguyen Thi Cuc, Chau Van Minh, Nguyen Thi Mai Phuong, Phan Van Kiem (2015) Biscembranoids from Vietnamese marine sponge Petrosia nigricans. Vietnam J Chem. 53(2e): 98-102.

4. Nguyen Thi Mai Phuong, Tran Thi Nhung, Nguyen Cong Thanh, Quach Thi Lien, Bach Nhu Quynh, Pham Van Lieu (2015) Ethyl acetate extract from (Rhodomyrtus tomentosa (Aliton) Hassk) leaves inhibits acid production and biofilm formation by Streptcocccus mutans. J Vietnam Med.

5. *Phuong Thi Mai Nguyen, Megan L. Falsetta, Geelsu Hwang, Mireya Gonzalez, and Hyun Koo (2014) -Mangostin Disrupts the Development of Streptococcus mutans Biofilms and Facilitates its Mechanical Removal. Plos One 9(10):e111312 doi: 10.1371/journal.pone.0111312.

6. *Nguyen Thi Mai Phuong, Trinh Tat Cuong, Dang Ngoc Quang (2014) Anti-inflammatory activity of methyl ferulate isolated from Stemona tuberosa Lour. Asian Pac J Trop Med. 7S1:S327-31.

7. Nguyen Thi Mai Phuong, Vo Hoai Bac, Pham Thi Luong Hang, Hoang Phuong Ha, Tran Thi Nhung (2014) Alpha-mangostin inhibits biofilm formation by Streptococcus mutans UA159. J. Biol. 35(3se): 100-105.

8. Ho Anh Son, Nguyen Linh Toan, Nguyen Thi Mai Phuong, Dao Chi Dung (2014) Proliferation of Bacillus subtilis HU58 in digestive track of mice. J. Arm. Med. Phar. 39(3): 30-38.

9. Nguyen Thi Mai Phuong, Hoang Phương Ha, Pham Thi Tra, Tran Thi Nhung (2013) Effect of some week acids and phytochemicals on malolactic fermentation by Streptococcus mutans. J. Biol. 35 (3): 376-382.

10. Tran Thi Nhung, Pham Thi Thu Phuong, Nguyen Thuy Hương, Nguyen Thi Mai Phuong (2013) Optimisation of rice bran hydolysation by xylanase for xylooligosacchride (XOS) production J. Biol. 35 (1): 67-73.

11. Nguyen Thi Mai Phuong, Trinh Tat Cuong, Tran Thi Nhung, Duong Thi Nu, Đang Ngoc Quang (2013) Establishment of in vitro model for screening of anti-inflammatory agents via toll like 4 (TLR4) on macrophage. J. Pharmacol. 53 (443): 5-10.

12. Ho Anh Son, Nguyen Linh Toan, Nguyen Thi Mai Phuong; Pham Thi Thu Phuong (2013) Evaluation of effectiveness of synbiotic on experimental diarrhea model. J. Arm. Pharmacol-Med. 5: 62-69.

13. Hoang P.H., Nguyen T.P., Tran T.N., Nguyen T.V.A, Nguyen H.A., Nguyen P.T.M (2013) Xylooligosachcharides (XOS) fermentation by Bacillus bacteria. J. Sci. Technol. 51 (1): 43-50.

14. Nguyen P.T.M., Le T.A., Phan T.N. (2012) H2O2 inhibits NADH oxidase activity purified from streptococcus mutans. J. Biotechnol. 9: 777-782.

15. Nguyen P.T.M., Marquis R.E. (2011) Antimicrobial activity against oral Streptococci of mangostin isolated from the peels of Garcinia mangostana L. J. Mater. Med. 16(5): 298-303.

16. Nguyen P.T.M., Marquis R.E. (2011) Malolactic fermentation by Streptoccocus mutans and protection against oxidative damage. J. Biol. 33(4): 92-96.Vietnamese

17. *Nguyen P.T.M., Marquis R.E. (2011) Antimicrobial actions of alpha-mangostin against oral Streptococci, Can. J. Microbiol. 57(3): 217-25.

18. Do T.T, Nguyen T.T., Nguyen N.H., Nguyen T.A.T., Phung V.T., Quyen D.T., Nguyen P.T.M., Nguyen T.N.D. (2010) Isolation and antibacterial activity of a-mangostin isolated from Garcinia mangostana L. Proceedings of the 35th anniversary of Vietnam Academy of Science and Technology foundation (1975-2010). pp. 136-143.

19. Tran T.T., Nguyen P.T.M., Phan T.N. (2010) Additional characterization of acid adaptation in Streptococcus mutans. J. Sci. 26(4): 647-655..

20. Nguyen P.T.M., Nguyen T.T., Nguyen D.L., Phan T.N. (2010) Bioactivity of Xanthone Preparation From The Peel of Mangosteen (Garcinia Magostana L.) J. Biotech. 3A (8): 717-725.

21. *Sheng J, Baldeck J.D, Nguyen P.T.M., Quivey Jr. R.G and Marquis R.E.(2010) Alkaline production associated with malolactic fermentation by oral streptococci and protection against acid , oxidative and starvation damage. Can. J. Microbiol 56(7):539-47.

22. Nguyen P.T.M. (2009) Methods to enhance damage to oral streptococci in suspension and biofilms by use of reduced transition-metal cations and chelators. Adv. Nat. Sci.10 (3): 111-116.

23. Nguyen T.T., Ngo T.H., Nguyen P.T.M. (2008) Preliminary study on response to oxidative stress promoted by menadione in Streptococcus mutans. Proceedings of 4th National Conference on Biochemistry and Molecular Biology in Agriculture, Medicine Biology and Food Industry Hanoi, Vietnam 16-17, October, 2008. pp. 131-133.

24. Nguyen P.T.M., Nguyen T.N.D., Do N.L. (2008) Chemical structure and antimicrobial action against Streptococcus mutans of GC13 isolated from the peels of Garcinia mangostana L . Proceedings of 4th National Conference on Biochemistry and Molecular Biology in Agriculture, Medicine Biology and Food Industry Hanoi, Vietnam 16-17, October, 2008. pp. 517-521.

25. Nguyen P.T.M., Nguyen T.T. (2008) Oxidative physiology in Streptococcus mutans under stress condition caused by 8-hydroxyquinoline. J. Biotechnol. 6(4): 483-488.

26. Nguyen T.T., Nguyen P.T.M. (2008) Preliminary study on response to oxidative stress in Streptococcus mutans . J. Biotechnol. 6(2): 197-202.

27. Nguyen P.T.M., Bui T.A. (2007) Investigation of antimicrobial activity against dental caries pathogen Streptococcus mutans of medicinal plants grown in Vietnam. J. Mater. Med. 1(12): 19-23.

28. Nguyen P.T.M., Trinh A.T. (2007) Adaptation of the vesicle assay for rapid screening of membrane interacting compounds from plants. J. Biol. 29(3): 72-77.

29. Nguyen P.T.M., Phan T.N., Marquis R.E. (2007) Physiological and biochemical characteristics of Streptococcus sobrinus 6715. J. Sci. 6715. 23: 181-187.

30. *Koo H. , Sheng J., Nguyen P.T.M., Marquis R.E. (2006) Cooperative inhibition by fluoride and zinc of glucosyltransferase production and polysaccharide synthesis by mutants Streptococci in suspension cultures and biofilms. FEMS Microbiol. Lett. 254(1):134-140.

31. Nguyen P.T.M., Phan T.N., Marquis R.E., (2006) Zinc effects on oxidative physiology of oral bacteria. Adv. Natural. Sci.7(1):31-138.

32. *Sheng J., Nguyen P.T.M., Baldeck J.D., Olsson J. and Marquis R.E., (2006) Antimicrobial actions of benzimidazoles against oral anaerobes Fusobacterium nucleatum and Prevotella intermedia. Arch. Oral. Biol., 51(11):1015-1023.

33. Nguyen P.T.M., Phan T.N., (2006) Zinc effects on enzymes of oral bacteria. J. Biol. 28(2): 86-90.

34. Nguyen P.T.M., and Marquis R.E., (2006) -mercaptoethanol can reverse the inhibition of glycolytic enzyme activities of Steptococcus mutans UA159 by benzimidazoles, J. Biol. 28(3): 66-70.

35. Nguyen P.T.M., Lilley M.R., Zhang R. (2005) Characterization of the response of three EMS mutans of Arabidopsis thaliana to arsenic stress. J. Biol. 2: 72-76.

36. *Nguyen P.T.M., Baldzac J.D., Olsson J., Marquis R.E (2005) Antimicrobial actions of benzimidazoles against oral bacteria. Oral Microbiol & Immunol. 20: 93-100.

37. *Sheng J., Nguyen P.T.M., Marquis R.E., (2005) Multi-target Antimicrobial actions of zinc against oral anaerobes. Arch. Oral. Biol., 50: 747-757.

38. Nguyen D.T., Phan T.N., Nguyen P.T.M., and Do N.T. (2004) Purification and characterization of superoxide dismutase of Streptococcus mutans. Proceedings of National Conference on Life Sciences, Hanoi, Vietnam 10/2004. pp. 217-223.

39. Nguyen P.T.M., Phan T.N., Nguyen T.N.D., Dang M.P., (2004) Effect of mangosteen extract on Streptococcus mutan. Proceedings of National Conference on Life Sciences, Hanoi, Vietnam 10/2004. pp. 337-341.

40. Nguyen P.T.M., Nguyen T.N.D., Do N.L., Marquis R.E. (2004) Killing augmentation of Streptococcus mutans in biofilms and suspensions by transition-metal cations and 8-hydroxyquinoline. J. Biol. 2: 31-36.

41. Nguyen P.T.M., Phan T.N., Do N.L., Nguyen T.N.D (2004) Polyphenols in the peels of Garcinia mangostana L and inhibitory effect on acid production by Streptococcus mutans GS-5. J. Pharmacol. 44:18-21.

42. Nguyen P.T.M., Lilley M.R., Zhang R. (2003) Screening of Arabidopsis thaliana T-DNA Insertion Mutans in Response to Arsenic Stress. J. Biol. 25(2): 13-18.

43. Nguyen P.T.M., Nguyen T.N.D., Do N.L., Marquis R.E. (2003) Augmentation of oxidative damage on Streptococcus mutans in dental plaque by 8-Hydroxyquinoline. J. Genetics & Appl. l2:18-23.

44. Nguyen P.T.M., Phan T.N., Marquis R.E. (2003) Effects of 8-Hydroxyquinoline on

physiological and biochemical processes of Streptococcus mutans UA159 in dental plaque. J. Pharmacol. 10:10-14.

45. Nguyen P.T.M., Nguyen T.N.D. (2003) Effects of Polyphenol in Green Tea Leaves (Camellia sinensis L.) on Streptococcus mutans. J. Mater. Med. 4: 110-114.

46. Nguyen P.T.M., Nguyen T.N.D., Vu T.M.D.(2003) Investigation of oral bacteria in Vietnamese dental plaque. J. Vietnam. Med. 8: 11-17.

47. Phan T.N., Nguyen P.T.M., Marquis R.E. (2003) Action mechanisms of hydrogen peroxide on Streptococcus mutans. J. Biol. 25(2a): 104-110.

48. Nguyen P.T.M., Lilley M.R., Zhang R. (2002) Molecular characterisation of arsenic accumulating mutants as1 and as2 of Arabidopsis thaliana. J. Sci. Appl. Biotech. 4: 39-45.

49. *Nguyen P.T.M., Abranches J., Phan T.N. and Marquis R.E. (2002) Repressed respiration of oral Streptoccoci grown in biofilms. Curr. Microbiol. 44: 262-266.

50. *Phan T.N., Nguyen P.T.M., Abranches J., Marquis R.E. (2002) Fluoride and organic weak acids as respiration inhibitors for oral Streptococci in acidified environments. Oral Microbiol. & Immunol. 17: 117-121.

51. Nguyen H.T., Nguyen P.T.M., Hoa T.H., Nguyen X.T., Le L.O. (1998) Effects of extracts from Kien co (Rhicanthus nasuutus (L) Kurl) on the growth and motality of insect Ppests, Plutella xylostella. J. Biol. 16(3) : 17-20.

52. Le L.O., Nguyen H.T., Nguyen P.T.M., Nguyen X.T., Hoa T.H. (1996) Effects of extracts from Bim Bim (Ipomoea hederacea JACQ) on rice insects. Annual Report of Institute of Biotechnology. 420-427 pp.

53. Nguyen T.K., Le L.O., Nguyen P.T.M. (1993) Preliminary studies on utilization of extracts from tropical plants for pest ontrol. Bulletin of Eco. Inst. NCST 1990-1992. 271-275 pp.

54. Do N.L., Nguyen P.T.M. (1992) Purification and characterization of a lectin from Sophora japonica L. J. Biol. 14(2): 12-15.

V. RESEARCH PRODUCTS

New functional food Synbiotic P&P containing prebiotic xylooligosaccharide (XOS) and probiotic Bacillus subtilis spores.